°®¶¹´«Ã½ Pharmacokinetics and Bioanalysis Facility
The overall goal of the PK core facility is to provide Absorption, Distribution, Metabolism, Excretion (ADME) and pharmacokinetic/pharmacodynamic services to support research in drug development and translational medicine.
The core facility is located at School of Pharmacy Research and Development (R&D) Center adjacent to the School of Pharmacy and Pharmaceutical Sciences (SOPPS) building. The facility is supported by a team of skilled research analysts and scientists with expertise in preclinical drug discovery and bioanalysis. The animal facility has more than 4,000 sq ft dedicated housing, procedure rooms, and experimental space.
Service Offerings for the Pharmacokinetics Facility
- In vivo animal studies
- Pharmacokinetic study in the rodents for small molecules, large molecules, and antibody-drug conjugates
- Oral bioavailability study
- Tissue distribution study
- Discovery Toxicology/Toxicokinetic study
- In vitro ADME studies
- Solubility and general stability
- Drug permeability
- Blood partitioning and protein binding
- Metabolic stability
- CYP inhibition, induction and reaction phenotyping
- In silico pharmacokinetic/pharmacodynamic modeling
- Non-compartmental and Compartmental pharmacokinetic analysis
- Physiologically based pharmacokinetic modeling and simulation
- Pharmacokinetic/pharmacodynamic modeling and simulation
Sevice Rates
| Service | Internal Rate | External Rate |
| LC-MS/MS analysis: Plasma/Urine Sample (precipitation) | $30 / sample | $45 / sample |
| LC-MS/MS analysis: Plasma/Urine Sample (SPE/LLE)* | To be approved | To be approved |
| LC-MS/MS analysis: New Compound Assay Development | To be approved | To be approved |
| Small molecule single dose PK study in the mouse | To be approved | To be approved |
| Small molecule single dose PK study in the rat | To be approved | To be approved |
| Pharmacokinetic analysis via non-compartmental analysis | To be approved | To be approved |
| PBPK , PK/PD analysis, modeling and simulation | To be approved | To be approved |
| Blood partitioning and plasma protein binding | To be approved | To be approved |
| In vitro permeability assay | To be approved | To be approved |
| Distek Dissolution Apparatus | $40/hour | $62.5/hour |
*, SPE: Solid-Phase Extraction; LLE: Liquid-Liquid Extraction.
For other ADME studies, including metabolic stability assays or pharmacokinetic evaluations of large molecules and antibody-drug conjugates (ADCs), please contact the facility directly to discuss your specific project requirements and to obtain a cost estimate.
Instrumentation
(1) Mass Spectrometers
Shimadzu triple quadrupole LCMS-8045 with UHPLC
The Shimadzu triple quadrupole LCMS-8045 includes a heated ESI probe, high-temperature heating block, heated desolvation line, drying gas, and focusing optics which all act to maximize sensitivity while minimizing contamination. The instrument is capable of providing accurate and stable data over long periods of time. The inclusion of Shimadzu's ultra-high-speed high-voltage power supply enables fast scan speed (30,000 u/s) and polarity switching time (5 ms).
Waters Xevo TQD triple quadrupole Mass Spectrometer with Acuity UPLC
Xevo TQD features T-Wave collision cell technology to provide the very best high-speed MRM and valuable, information-rich acquisition mode known as RADAR. The system incorporates Intellistart Technology, for automated system optimization and status monitoring. Ensuring that the highest quality data for chemical detection.
Waters Acquity UPLC (Hâ€class) with QDa quadrupole MS and fluorescence detector
The Waters Acquity UPLC H-Class-QDa System comes with a quaternary solvent manager, an autosampler, a PDA detector, and a single quadrupole QDa mass detector, facilitating the concurrent acquisition of MS (Selected Ion Recording and/or full scan) and PDA data. Ideal for the rapid characterization of synthetic chemical entities (low resolution) and routine quantitative assessments, this system provides a comprehensive solution for analytical needs.
Orbitrap Mass Spectrometer (Qâ€Exactive HF)
The Thermo Scientificâ„¢ Q Exactiveâ„¢ HF hybrid quadrupole-Orbitrap mass spectrometer features an ultra-high-field Orbitrap analyzer and utilizes the same Active Beam Guide technology and Advanced Quadrupole design for very stable system operation and exceptional analytical performance. The Q Exactive HF instrument can be used in a variety of pharmacokinetic and pharmacodynamic applications. The system is very suitable for characterization of small polypeptides and small molecule drugs using Data dependent acquisition as well as targeted quantification using of molecules high resolution parallel reaction monitoring (PRM). The system is also highly suitable for characterization of biologic drugs and peptide mapping as well as covalent drug-protein interactions.
Other instruments
IVIS® Lumina III In Vivo Imaging System (PerkinElmer)
PIXITM automated immunoassay platform
Ellaâ„¢ Automated ELISA
Other instruments: BioRad CFX384 and CFX96 RTâ€PCR systems, BD flowcytometry sorter, Leica confocal microscope, Ella Automated Immunoassay System (Biotechne), tissue homogenizer, Digital droplet qPCR, etc.
Personnel
Tao Zhang, PhD, Director
Associate Professor of Pharmaceutical Sciences
Dr. Zhang has extensive industrial and academic experience in preclinical drug absorption, distribution, metabolism and elimination (ADME) study, in vitro and in vivo extrapolation (IVIVE), pharmacokinetic/ pharmacodynamic (PK/PD) modeling and physiologically based pharmacokinetic (PBPK) modeling and simulation.
Office: PB422
Phone: 607-777-5822 (O)
Email: zhangt@binghamton.edu
Mohammad Asikur Rahman, Ph.D.
Scientist, Postdoctoral Fellow
Sung Hun Bae, Ph.D.
Scientist, Postdoctoral Fellow
Bofang Yi, M.S.
Scientist, Research Assistant
Affiliated Faculty
Nathan Tumey, Ph.D.
Associate Professor, Pharmaceutical Sciences
Dr. Tumey is an expert in antibody-drug-conjugates (ADCs) bioanalysis and application of ADCs for the treatment of auto-immune disorders and rare diseases.
Yetrib Hathout, Ph.D.
Professor, Pharmaceutical Sciences
Dr. Hathout is an expert in developing and standardizing innovative mass spectrometry and proteomics methods for biomarker studies and for understanding the molecular mechanism of diseases.